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Which step in the creation of cDNA involves the use of reverse transcriptase? Which step in the creation of cDNA involves the use of reverse transcriptase?   A) step 1 B) step 2 C) step 3 D) step 4


A) step 1
B) step 2
C) step 3
D) step 4

E) All of the above
F) None of the above

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C

The number of proteins in humans


A) is much greater than the number of genes.
B) is approximately equal to the number of genes.
C) cannot be determined because the human genome is too complex.
D) is less than half the number of genes.

E) B) and D)
F) A) and B)

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Which of the following statements about genomics is false?


A) The first complete genome to be sequenced was a prokaryote.
B) DNA technology limits genomic studies to prokaryotes.
C) Genes from different species that have analogous sequences suggest similar function.
D) Whole sets of genes and their interactions are studied in the field of genomics.

E) A) and B)
F) A) and C)

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The type of recombinant bacteria most often used to mass- produce genes is


A) Pseudomonas aeruginosa.
B) Escherichia coli.
C) Agrobacterium tumefaciens.
D) Saccharomyces cerevisiae.

E) A) and B)
F) A) and C)

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The polymerase chain reaction relies upon unusual,heat- resistant _ that were isolated from bacteria living in hot springs.


A) DNA polymerase molecules
B) restriction enzymes
C) phages
D) plasmids

E) None of the above
F) All of the above

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What is the preferred name of the technique used to determine if DNA comes from a particular individual?


A) DNA outline
B) DNA scrutiny
C) DNA fingerprinting
D) DNA profiling

E) A) and C)
F) B) and C)

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Genomic libraries can be constructed using either bacterial plasmids or what other vector?


A) human chromosomes
B) bacteriophages
C) tRNA
D) ribosomes

E) A) and B)
F) C) and D)

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Segments of eukaryotic DNA that can move or be copied from one site to another in the genome are called


A) exons.
B) transposable elements.
C) vectors.
D) plasmids.

E) B) and C)
F) C) and D)

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A nucleic acid probe is


A) a virus that transfers DNA to a recipient cell.
B) a piece of radioactively labeled DNA that is used to locate a specific gene.
C) a plasmid that recognizes a specific DNA sequence.
D) an enzyme that locates a specific restriction site on RNA.

E) None of the above
F) C) and D)

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Why is the whole- genome shotgun method currently the tool of choice for analyzing genomes?


A) It is very labor- intense.
B) It is extremely accurate.
C) Multiple genomes can be analyzed simultaneously.
D) It is fast and inexpensive.

E) B) and C)
F) A) and D)

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The enzyme that converts information stored in their RNA to information stored in DNA is


A) reverse transcriptase.
B) a restriction enzyme.
C) RNA polymerase.
D) DNA ligase.

E) B) and D)
F) C) and D)

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Which of the following statements about DNA technology is false?


A) DNA technology is now used to produce vaccines that are harmless mutants of a pathogen.
B) DNA technology is now used to mass- produce human growth hormone.
C) DNA technology is now used to mass- produce human insulin.
D) DNA technology is now used to create cells that can identify and kill cancer cells.

E) A) and B)
F) C) and D)

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D

Which of the following statements regarding proteomics is correct?


A) Proteomics and genomics allow scientists to study life in an ever- increasing reductive approach.
B) Proteomics is the systematic study of the full set of proteins encoded by a genome.
C) Proteomics is the study of protein interaction within a cell.
D) Proteomics involves the complete analysis of the prokaryotes.

E) B) and D)
F) A) and D)

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In order for gene therapy to be permanent,


A) the defective gene must first be removed from all somatic cells.
B) the normal gene must be added to the germ line cells.
C) the normal gene must be transferred to somatic cells that can continuously multiply.
D) the normal gene must first be treated with UV radiation to ensure noninfectivity.

E) B) and D)
F) None of the above

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When plasmids are used to produce a desired protein,


A) the desired gene is inserted into the plasmid and the plasmid is returned to the bacterium by transformation.
B) the plasmids are inserted into the bacterial chromosome.
C) the bacterial genome and plasmid are inserted into the genome of the cell containing the desired gene (often the cell of a plant or animal) .
D) the plasmids multiply and produce the protein outside of the bacterium.

E) All of the above
F) A) and B)

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Golden rice is golden in color because it is rich in


A) vitamin C.
B) chromium picolinate.
C) vitamin A.
D) beta- carotene.

E) C) and D)
F) A) and B)

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Gel electrophoresis sorts DNA molecules on the basis of their


A) solubility in the gel.
B) ability to bind to mRNA.
C) size.
D) nucleotide sequence.

E) A) and B)
F) B) and D)

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Which of the following statements regarding DNA is false?


A) Telomeres seem to have a structural function.
B) Scientists think that the typical human gene specifies just one polypeptide.
C) Long stretches of repetitive DNA are prominent at centromeres and ends of chromosomes.
D) Current estimates are that there are 20,000 genes in the human genome.

E) B) and C)
F) A) and C)

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B

Which of the following statements about genome sequencing is false?


A) Most of the genomes that have been sequenced to date are eukaryotes.
B) The first eukaryotic organism to have its genome sequenced was yeast.
C) The genomes of over 1,000 species have been completely or almost completely sequenced.
D) The genome of a mouse has been sequenced.

E) B) and D)
F) B) and C)

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Which of the following statements regarding repetitive DNA is false?


A) Repetitive DNA can show great variation among individuals.
B) Repetitive DNA is identical in all humans.
C) Repetitive DNA is usually repeated multiple times in the genome.
D) Repetitive DNA is usually found between genes.

E) None of the above
F) A) and D)

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