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No eukaryotic organisms have been shown to fix nitrogen without the aid of prokaryotes.

A) True
B) False

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Oligotrophic waters are usually hypoxic.

A) True
B) False

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Biofilms are likely to be found in environments


A) that are nutrient rich.
B) that are relatively warm.
C) with rapidly flowing water.
D) with low nutrients.

E) B) and D)
F) B) and C)

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The organisms that convert carbon dioxide into organic matter are the


A) secondary consumers.
B) primary consumers.
C) producers.
D) decomposers.

E) None of the above
F) B) and D)

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Which of the following soil organisms play a major role in decomposing plant matter?


A) bacteria
B) fungi
C) protozoa
D) viruses
E) bacteria AND fungi

F) A) and E)
G) A) and C)

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In many aquatic habitats, the growth of algae and cyanobacteria is limited by the amount of


A) nitrogen.
B) calcium.
C) sulfur.
D) phosphorus.

E) A) and B)
F) A) and C)

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Next to carbon and oxygen, the element organisms most require is


A) sulfur.
B) phosphorus.
C) nitrogen.
D) iron.

E) B) and C)
F) None of the above

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How can the biomass of fungi in soil be greater considering that bacteria are more numerous?


A) Fungi are always multicellular organisms, while bacteria are unicellular. As such, biomass of fungi would always be larger than that of bacteria, since they possess more cells in each individual organism.
B) This is a matter of size. Fungi, whether unicellular or multicellular, are eukaryotic. As such, they will generally be larger than bacteria (which are prokaryotic) . This means that, even with a lower overall number, they will have more total biomass.
C) It depends on where they grow. Fungi generally grow in the most moist areas near the top of soil. This gives them an advantage over bacteria. They may not be as numerous, but this advantage in location and nutrients is what makes them larger than the bacteria (and therefore having more biomass) . If the bacteria evolved to take over the top portion of the soil, THEY would have the larger biomass than the fungi.
D) Fungi are capable of incorporating the dead materials around them into themselves as nutrient sources (while bacteria are not) . This capacity allows them to greatly increase their biomass per each organism, well beyond what a simple bacterial cell could hold. This capability is what allows them to achieve higher biomass than bacteria.

E) All of the above
F) C) and D)

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The problem of not being able to cultivate most microorganisms has led to the use of


A) FISH.
B) microscopy with fluorescent dyes.
C) 16s rRNA analysis.
D) DGGE.
E) All of these methods help identify microorganisms in the environment.

F) B) and D)
G) C) and D)

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The bacteria cultured from a soil sample typically represents


A) all the bacteria present in that sample.
B) a very small number of bacteria present in that sample.
C) all the metabolically active bacteria.
D) all the metabolically inactive bacteria.

E) B) and C)
F) A) and B)

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Streptomyces produces geosmins which give soil a characteristic musty odor.

A) True
B) False

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Fungi are able to handle acidic environments much better than bacteria.

A) True
B) False

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Streptomyces


A) may produce antibiotics.
B) may produce geosmins.
C) will swarm together to form fruiting bodies.
D) are typically found in the soil.
E) may produce antibiotics, may produce geosmins AND are typically found in the soil.

F) A) and E)
G) D) and E)

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A microbial mat


A) is a form of biofilm.
B) is poorly organized.
C) contains only obligate anaerobes.
D) is constructed to prevent interactions between the various layers of microorganisms.
E) is a form of biofilm AND is poorly organized.

F) None of the above
G) A) and E)

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During aerobic decomposition of organic matter the primary gas produced is


A) oxygen.
B) hydrogen sulfide.
C) nitrogen.
D) carbon dioxide.

E) A) and D)
F) B) and D)

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The organisms isolated from hydrothermal vents are typically


A) phototrophs.
B) chemolithoautotrophs.
C) rhizobia.
D) bacteroid.
E) phototrophs, rhizobia AND bacteroid.

F) All of the above
G) A) and E)

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Which group is composed of autotrophs?


A) primary producers
B) primary consumers
C) secondary consumers
D) decomposers

E) None of the above
F) C) and D)

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A mycorrhizae is a symbiotic association between roots and fungi.

A) True
B) False

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Nutrient poor waters are described as being


A) hypoxic.
B) oligotrophic.
C) autotrophic.
D) eutrophic.

E) A) and D)
F) A) and B)

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The zone of soil that surrounds the roots and contains a variety of organic exudates is called the


A) mycorrhizae.
B) rootsphere.
C) rhizosphere.
D) geosmin.

E) All of the above
F) A) and B)

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