Question 1

Cells that contain a "blue/white screening" plasmid that has an added gene are recognized by the method:

Accepted Answer

A)   Ability to grow on ampicillin.
B)   Inability to grow on ampicillin.
C)   The colonies have a blue color.
D)   The colonies lack a blue color.
E)   More than one of these choices indicates that the plasmid contains recombined DNA.F) A) and B)
G) B) and E)

Question 2

Genetic recombination occurs in nature.

Accepted Answer

A) True 
 B)False

Question 3

In gene therapy

Accepted Answer

A)   restriction enzymes are introduced into cells to cleave specific genes.
B)   new origins of replication are added to cells to promote cell division.
C)   cells of specific tissues are altered in a way that alleviates the effects of a disease.
D)   traits are altered so that descendents of those treated will inherit those new traits.E) B) and C)
F) A) and B)

Question 4

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

Accepted Answer

A)   Erythropoietin
B)   Human Growth Hormone
C)   Insulin
D)   Enterokinase
E)   Erythropoietin and Human Growth HormoneF)  All of theseG) A) and D)
H) B) and F)

Question 5

A palindrome is

Accepted Answer

A)   a DNA sequence that contains only one kind of base
B)   a DNA sequence that contains only two kinds of bases
C)   a sequence that reads the same from left to right or from right to left
D)   none of the aboveE) A) and B)
F) B) and C)

Question 6

A proteome is

Accepted Answer

A)   a protein-based vector.
B)   a three-dimensional protein structure.
C)   a collection of all the proteins produced in a given cell or tissue.
D)   an improperly digested protein responsible for certain diseases such as "mad cow" disease.E) A) and B)
F) B) and D)

Question 7

Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs.The b-gal gene codes for the enzyme b-galactosidase,the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.
R gene confers ampicillin resistance.   Refer to Exhibit 13D.Neglecting any discussion of whether it's a good or bad choice,I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid.How can I tell which transformants have the insert? A)  The bacteria will not be able to grow in the presence of ampicillin, and they will be blue. B)  The bacteria will not be able to grow in the presence of ampicillin, and they will be white. C)  The bacteria will be able to grow in the presence of ampicillin, and they will be blue. D)  The bacteria will be able to grow in the presence of ampicillin, and they will be white." class="answers-bank-image d-inline" rel="preload" > Refer to Exhibit 13D.Neglecting any discussion of whether it's a good or bad choice,I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid.How can I tell which transformants have the insert?

Accepted Answer

A)   The bacteria will not be able to grow in the presence of ampicillin, and they will be blue.
B)   The bacteria will not be able to grow in the presence of ampicillin, and they will be white.
C)   The bacteria will be able to grow in the presence of ampicillin, and they will be blue.
D)   The bacteria will be able to grow in the presence of ampicillin, and they will be white.E) A) and D)
F) B) and C)

Question 8

The "natural" function of restriction endonucleases is to

Accepted Answer

A)   protect bacterial cells from invasion by viruses (bacteriophages) .
B)   help bacteriophages infect cells.
C)   regulate gene expression from specific promoters.
D)   remove chromatin from histones.E) None of the above
F) All of the above

Question 9

The "c" in cDNA stands for this word:

Accepted Answer

A)   Complete.
B)   Circular.
C)   Complementary.
D)   Chromosomal.
E)   Confusing.F) D) and E)
G) C) and E)

Question 10

Recombinant DNA molecules contain covalently linked segments derived from two or more sources.

Accepted Answer

A) True 
 B)False

Question 11

Which of the following is not required in order for a plasmid to be used in genetic recombination?

Accepted Answer

A)   An "ori" site.
B)   A gene to allow for easy screening an isolation of cells which contain the plasmid.
C)   A poly-cloning site or multi-cloning site for gene insertion.
D)   At least one site for a restriction enzyme to cut.
E)   All of these are essential in a plasmid for DNA recombination.F) None of the above
G) B) and D)

Question 12

How is human insulin produced by genetically engineered bacteria?

Accepted Answer

A)   DNA that codes for each of the two polypeptide chains is introduced into two different populations of bacteria
B)   DNA that codes for the insulin precursor is introduced into bacterial plasmids
C)   DNA that codes for both the polypeptide chains is introduced into a single population of bacteria
D)   This procedure cannot be done.E) A) and C)
F) B) and C)

Question 13

The polymerase chain reaction requires

Accepted Answer

A)   primers complementary to the ends of the sequence to be amplified
B)   careful temperature control
C)   both of these
D)   neither of theseE) A) and C)
F) A) and B)

Question 14

Which of the following plants have been genetically modified?

Accepted Answer

A)   Soy beans
B)   Corn.
C)   Cotton
D)   Tomatoes
E)   All of these plants have been genetically modified.F) A) and B)
G) B) and C)

Question 15

A multiple clonic site is also known as:

Accepted Answer

A)   a polylinker
B)   an origin of replication
C)   a restriction enzyme site
D)   a selectable markerE) A) and B)
F) A) and C)

Question 16

If mRNA is converted to DNA,digested with restriction enzymes,and cloned into suitable vectors,the resultant library is called a:

Accepted Answer

A)   DNA library
B)   cDNA library
C)   mRNA library
D)   none of theseE) All of the above
F) A) and C)

Question 17

Which of the following are methods used to determine where the DNA bands are located on an electrophoresis gel?

Accepted Answer

A)   Radioactivity
B)   Fluorescence
C)   Dyes which bind to DNA
D)   Luminescence
E)   All of these can visualize the DNAF) D) and E)
G) A) and B)

Question 18

When fluorescent markers are used for DNA sequencing:

Accepted Answer

A)   the DNA separations are faster
B)   four different reaction mixtures must be used
C)   computer analysis can help in the detection of sequencing analysis at a faster rate
D)   none of theseE) None of the above
F) All of the above

Question 19

Fluorescence works because the fluorescent molecule

Accepted Answer

A)   absorbs light at one wavelength and emits light at a longer wavelength.
B)   absorbs light at one wavelength and emits light at a shorter wavelength.
C)   absorbs light at one wavelength and emits light at the same wavelength.
D)   absorbs light at many wavelengths and emits light at many wavelengths.E) C) and D)
F) B) and D)

Question 20

The use of the Bt gene is prohibited in corn plants.

Accepted Answer

A) True 
 B)False

Exam 13: Nucleic Acid Biotechnology Techniques

Which of the following plants have been genetically modified?

Correct Answer
verified

Which of the following are methods used to determine where the DNA bands are located on an electrophoresis gel?

Correct Answer
verified

If mRNA is converted to DNA,digested with restriction enzymes,and cloned into suitable vectors,the resultant library is called a:

Correct Answer
verified

A multiple clonic site is also known as:

Correct Answer
verified

A proteome is

Correct Answer
verified

Correct Answer
verified

Which of the following is not required in order for a plasmid to be used in genetic recombination?

Correct Answer
verified

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

Correct Answer
verified

When fluorescent markers are used for DNA sequencing:

Correct Answer
verified

The use of the Bt gene is prohibited in corn plants.

Correct Answer
verified

Recombinant DNA molecules contain covalently linked segments derived from two or more sources.

Correct Answer
verified

Fluorescence works because the fluorescent molecule

Correct Answer
verified

Cells that contain a "blue/white screening" plasmid that has an added gene are recognized by the method:

Correct Answer
verified

The polymerase chain reaction requires

Correct Answer
verified

The "c" in cDNA stands for this word:

Correct Answer
verified

Genetic recombination occurs in nature.

Correct Answer
verified

How is human insulin produced by genetically engineered bacteria?

Correct Answer
verified

In gene therapy

Correct Answer
verified

The "natural" function of restriction endonucleases is to

Correct Answer
verified

A palindrome is

Correct Answer
verified

Exam 13: Nucleic Acid Biotechnology Techniques

Which of the following plants have been genetically modified?

Correct AnswerverifiedShow Answer

Which of the following are methods used to determine where the DNA bands are located on an electrophoresis gel?

Correct AnswerverifiedShow Answer

If mRNA is converted to DNA,digested with restriction enzymes,and cloned into suitable vectors,the resultant library is called a:

Correct AnswerverifiedShow Answer

A multiple clonic site is also known as:

Correct AnswerverifiedShow Answer

A proteome is

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which of the following is not required in order for a plasmid to be used in genetic recombination?

Correct AnswerverifiedShow Answer

Which of the following proteins are produced by recombinant DNA technology but have had their purposes subverted for improving athletic perforrmance?

Correct AnswerverifiedShow Answer

When fluorescent markers are used for DNA sequencing:

Correct AnswerverifiedShow Answer

The use of the Bt gene is prohibited in corn plants.

Correct AnswerverifiedShow Answer

Recombinant DNA molecules contain covalently linked segments derived from two or more sources.

Correct AnswerverifiedShow Answer

Fluorescence works because the fluorescent molecule

Correct AnswerverifiedShow Answer

Cells that contain a "blue/white screening" plasmid that has an added gene are recognized by the method:

Correct AnswerverifiedShow Answer

The polymerase chain reaction requires

Correct AnswerverifiedShow Answer

The "c" in cDNA stands for this word:

Correct AnswerverifiedShow Answer

Genetic recombination occurs in nature.

Correct AnswerverifiedShow Answer

How is human insulin produced by genetically engineered bacteria?

Correct AnswerverifiedShow Answer

In gene therapy

Correct AnswerverifiedShow Answer

The "natural" function of restriction endonucleases is to

Correct AnswerverifiedShow Answer

A palindrome is

Correct AnswerverifiedShow Answer

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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