Question 1

In analyzing microarray data if the ratio of mRNA present before and after a particular treatment is 1 it indicates that

Accepted Answer

A)  there was no change in mRNA levels before and after treatment.
B)  the gene was not expressed either before or after treatment.
C)  gene expression could not be detected.
D)  mRNA was not detected before treatment.E) A) and B)
F) A) and C)

Question 2

What can gene chips be used for?

Accepted Answer

A)  To examine patterns of protein expression under various environmental conditions
B)  To establish levels of genetic variation
C)  To determine the mass of a gene
D)  To identify the amount of a specific transcription factor in a cell.E) A) and D)
F) None of the above

Question 3

_______ can be used to determine the amino acid sequence of a protein or peptide.

Accepted Answer

A)  X-ray crystallography
B)  Nuclear magnetic resonance
C)  Mass spectrometry
D)  The ChIP assayE) B) and C)
F) A) and D)

Question 4

What is a reason that the proteome is larger than the genome?

Accepted Answer

A)  Alternative splicing
B)  Proteins are constantly being synthesized and degraded.
C)  Each protein has a specific function which are quite varied while the genes in the genome are only transcribed
D)  Each protein has multiple functional domains whille each gene consists of three basic parts.E) A) and C)
F) All of the above A

Question 5

The genes of a gene family that consist of two or more homologous genes are called ___.

Accepted Answer

A)  Orthologs
B)  Paralogs
C)  Polylogs
D)  HomologsE) A) and B)
F) B) and C)

Question 6

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by ____.

Accepted Answer

A)  amino acid sequence
B)  molecular mass
C)  abundance
D)  isoelectric pointE) C) and D)
F) All of the above

Question 7

To identify a functional protein motif that might be shared by several different proteins what type of recognition strategy would most likely be used?

Accepted Answer

A)   Pattern recognition
B)   Sequence recognition
C)   Identity recognition
D)   Search-by-contentE) All of the above
F) C) and D)

Question 8

cDNA uses chromosomal DNA as its template.

Accepted Answer

A) True 
 B)False

Question 9

A population of cells is treated with a polypeptide hormone that induces mitosis and the hormone's cell surface receptor is isolated after 15 minutes and subjected to fragmentation and mass spectometry. Several of the peaks show a slight shift as compared to the same protein from cells not treated with the hormone. What is the most likely explanation of this phenomenon?

Accepted Answer

A)   Phosphorylation of some amino acid residues.
B)   mRNA editing resulting in a slightly different amino acid sequence.
C)   Alternative exon usage.
D)   Proteolytic processing.E) B) and C)
F) B) and D)

Question 10

What technique is used to separate cellular proteins for analysis?

Accepted Answer

A)  Western blots
B)  Antibody microarrays
C)  Two-dimensional gel electrophoresis
D)  Functional protein microarraysE) A) and D)
F) B) and C)

Question 11

Genetic sequences may be useful in predicting protein structure because

Accepted Answer

A)   there is a high statistical frequency of specific amino acids within a given secondary structure
B)   the folding of the protein is based on how the mRNA folds.
C)   the physical and energetic properties of the DNA influence protein folding.
D)   knowing what specific codons are used for each amino acid in the protein can help predict its structure.E) A) and C)
F) C) and D) A

Question 12

Which technique can be used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Accepted Answer

A)  DNA microarray
B)  Treatment with reverse transcriptase to isolate the DNA of interest
C)  BLAST analysis
D)  Mass spectrometryE) A) and D)
F) All of the above

Question 13

Gene knockouts in mice can be useful because

Accepted Answer

A)  they can be used to develop models of human disease.
B)  they identify DNA binding proteins
C)  they limit mRNA production both from the knockout as well as the normal allele in diploids.
D)  only genes that are actively transcribed in the embryo are susceptible to being knocked out.E) C) and D)
F) B) and C)

Question 14

What field of study of genetics applies statistics and mathematical tools to genetic sequences in order to better understand the genetic information?

Accepted Answer

A)  Structural genomics
B)  Functional genomics
C)  Proteomics
D)  BioinformaticsE) None of the above
F) All of the above

Question 15

In two dimensional gel electrophoresis, proteins are first treated with SDS, separated according to molecular mass, and then separated by their net charge at a specific pH.

Accepted Answer

A) True 
 B)False

Question 16

In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by ____.

Accepted Answer

A)  net charge
B)  molecular mass
C)  abundance
D)  isoelectric pointE) B) and C)
F) A) and D)

Question 17

Two genes that share a common ancestral gene will probably have significant homology.

Accepted Answer

A) True 
 B)False

Question 18

What type of computer based search methodology would most likely be used to identify a potential promoter for a gene in a nucleotide sequence?

Accepted Answer

A)   Search-by-signal
B)   Search-by-content
C)   Search-by-identity
D)   Search-by-recognitionE) All of the above
F) B) and C)

Question 19

A functional protein microarray can be used to establish the function or substrates for a given group of proteins.

Accepted Answer

A) True 
 B)False

Question 20

What would be the most reasonable technique to use to determine if a drug is capable of inhibiting a transcription factor?

Accepted Answer

A)   ChIP assay used with a DNA microarray
B)   Mass spectometry of proteins in the cell
C)   2-D gel electrophoresis
D)   Antibody microarrayE) A) and B)
F) B) and C) A

Exam 23: Genomics II: Functional Genomics, Proteomics, and Bioinformatics

In analyzing microarray data if the ratio of mRNA present before and after a particular treatment is 1 it indicates that

Correct Answer
verified

What can gene chips be used for?

Correct Answer
verified

_______ can be used to determine the amino acid sequence of a protein or peptide.

Correct Answer
verified

What is a reason that the proteome is larger than the genome?

Correct Answer
verified
A

The genes of a gene family that consist of two or more homologous genes are called ___.

Correct Answer
verified

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by ____.

Correct Answer
verified

To identify a functional protein motif that might be shared by several different proteins what type of recognition strategy would most likely be used?

Correct Answer
verified

cDNA uses chromosomal DNA as its template.

Correct Answer
verified

Correct Answer
verified

What technique is used to separate cellular proteins for analysis?

Correct Answer
verified

Genetic sequences may be useful in predicting protein structure because

Correct Answer
verified
A

Which technique can be used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Correct Answer
verified

Gene knockouts in mice can be useful because

Correct Answer
verified

What field of study of genetics applies statistics and mathematical tools to genetic sequences in order to better understand the genetic information?

Correct Answer
verified

In two dimensional gel electrophoresis, proteins are first treated with SDS, separated according to molecular mass, and then separated by their net charge at a specific pH.

Correct Answer
verified

In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by ____.

Correct Answer
verified

Two genes that share a common ancestral gene will probably have significant homology.

Correct Answer
verified

What type of computer based search methodology would most likely be used to identify a potential promoter for a gene in a nucleotide sequence?

Correct Answer
verified

A functional protein microarray can be used to establish the function or substrates for a given group of proteins.

Correct Answer
verified

What would be the most reasonable technique to use to determine if a drug is capable of inhibiting a transcription factor?

Correct Answer
verified
A

Exam 23: Genomics II: Functional Genomics, Proteomics, and Bioinformatics

In analyzing microarray data if the ratio of mRNA present before and after a particular treatment is 1 it indicates that

Correct AnswerverifiedShow Answer

What can gene chips be used for?

Correct AnswerverifiedShow Answer

_______ can be used to determine the amino acid sequence of a protein or peptide.

Correct AnswerverifiedShow Answer

What is a reason that the proteome is larger than the genome?

Correct AnswerverifiedA

The genes of a gene family that consist of two or more homologous genes are called ___.

Correct AnswerverifiedShow Answer

In a two-dimensional gel electrophoresis experiment, proteins are separated horizontally by ____.

Correct AnswerverifiedShow Answer

To identify a functional protein motif that might be shared by several different proteins what type of recognition strategy would most likely be used?

Correct AnswerverifiedShow Answer

cDNA uses chromosomal DNA as its template.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

What technique is used to separate cellular proteins for analysis?

Correct AnswerverifiedShow Answer

Genetic sequences may be useful in predicting protein structure because

Correct AnswerverifiedA

Which technique can be used to identify a protein of interest after a two-dimensional gel electrophoresis experiment?

Correct AnswerverifiedShow Answer

Gene knockouts in mice can be useful because

Correct AnswerverifiedShow Answer

What field of study of genetics applies statistics and mathematical tools to genetic sequences in order to better understand the genetic information?

Correct AnswerverifiedShow Answer

In two dimensional gel electrophoresis, proteins are first treated with SDS, separated according to molecular mass, and then separated by their net charge at a specific pH.

Correct AnswerverifiedShow Answer

In a two-dimensional gel electrophoresis experiment, proteins are generally separated vertically by ____.

Correct AnswerverifiedShow Answer

Two genes that share a common ancestral gene will probably have significant homology.

Correct AnswerverifiedShow Answer

What type of computer based search methodology would most likely be used to identify a potential promoter for a gene in a nucleotide sequence?

Correct AnswerverifiedShow Answer

A functional protein microarray can be used to establish the function or substrates for a given group of proteins.

Correct AnswerverifiedShow Answer

What would be the most reasonable technique to use to determine if a drug is capable of inhibiting a transcription factor?

Correct AnswerverifiedA

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