Question 1

The host cell protein thioredoxin binds to the DNA polymerase of bacteriophage T7 and improves which of the following?

Accepted Answer

A)   speed
B)   accuracy
C)   processivity
D)   Both a and b are correct
E)   Both a and c are correct.F) A) and E)
G) B) and C)

Question 2

Bacteriophage T7 was isolated from what source?

Accepted Answer

A)   Unprocessed waste from a sewage treatment plant
B)   Commercially available treatment for intestinal infections
C)   Soil found on a farm
D)   Water samples taken from a polluted river
E)   Animal manure.F) D) and E)
G) B) and E)

Question 3

Describe how the RNA polymerase from bacteriophage T7, along with specific DNA sequences taken from the late T7 genes, can be used to produce high levels of a foreign protein in a bacterial cell.

Accepted Answer

The T7 RNA polymerase specifically recog...

Question 4

Holin is a small membrane-specific protein produced by the bacteriophge T7.Which of the following describes the function of this protein?

Accepted Answer

A)   It ensures the replication of the entire T7 genome.
B)   It helps the T7 phage particles lyse the host cell.
C)   It creates the portal in the phage head where the viral genome is packaged.
D)   It degrades the host cell wall during entry of the viral genome.
E)   It cleaves the viral genome during packaging.F) A) and E)
G) B) and D)

Question 5

The promoters of the Class II and Class III genes in the bacteriophage T7 have the same -10 and -35 sequences as the promoters for host cell genes.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following enzymes is NOT involved in replication of the bacteriophage T7 genome?

Accepted Answer

A)   Single-Strand DNA binding protein
B)   Lysozyme
C)   DNA ligase
D)   RNA polymease
E)   RNA primase/helicaseF) D) and E)
G) A) and C)

Question 7

What would be observed in a cell infected with a T7 phage mutated in gene 3 (endonuclease gene) .

Accepted Answer

A)   No Class II mRNAs.
B)   No Class III mRNAs.
C)   Incomplete entry of the viral genome.
D)   Concatamers of the viral genome.
E)   Incomplete virus capsids.F) A) and D)
G) B) and E)

Exam 7: Bacteriophage

The host cell protein thioredoxin binds to the DNA polymerase of bacteriophage T7 and improves which of the following?

Correct Answer
verified

Bacteriophage T7 was isolated from what source?

Correct Answer
verified

What would be observed in a cell infected with a T7 phage mutated in gene 3 (endonuclease gene) .

Correct Answer
verified

Describe how the RNA polymerase from bacteriophage T7, along with specific DNA sequences taken from the late T7 genes, can be used to produce high levels of a foreign protein in a bacterial cell.

Correct Answer
verified
The T7 RNA polymerase specifically recog...
View Answer

The promoters of the Class II and Class III genes in the bacteriophage T7 have the same -10 and -35 sequences as the promoters for host cell genes.

Correct Answer
verified

Which of the following enzymes is NOT involved in replication of the bacteriophage T7 genome?

Correct Answer
verified

Holin is a small membrane-specific protein produced by the bacteriophge T7.Which of the following describes the function of this protein?

Correct Answer
verified

Exam 7: Bacteriophage

The host cell protein thioredoxin binds to the DNA polymerase of bacteriophage T7 and improves which of the following?

Correct AnswerverifiedShow Answer

Bacteriophage T7 was isolated from what source?

Correct AnswerverifiedShow Answer

What would be observed in a cell infected with a T7 phage mutated in gene 3 (endonuclease gene) .

Correct AnswerverifiedShow Answer

Describe how the RNA polymerase from bacteriophage T7, along with specific DNA sequences taken from the late T7 genes, can be used to produce high levels of a foreign protein in a bacterial cell.

Correct AnswerverifiedThe T7 RNA polymerase specifically recog...View AnswerShow Answer

The promoters of the Class II and Class III genes in the bacteriophage T7 have the same -10 and -35 sequences as the promoters for host cell genes.

Correct AnswerverifiedShow Answer

Which of the following enzymes is NOT involved in replication of the bacteriophage T7 genome?

Correct AnswerverifiedShow Answer

Holin is a small membrane-specific protein produced by the bacteriophge T7.Which of the following describes the function of this protein?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The T7 RNA polymerase specifically recog...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified