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Which two organisms should be used to determine the validity of the nitrate reduction test?


A) N.gonorrhoeae and N.lactamica
B) Neisseria mucosa and M.catarrhalis
C) N.lactamica and M.catarrhalis
D) Neisseria sicca and Neisseria subflava

E) A) and B)
F) None of the above

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M.catarrhalis may be described as:


A) oxidase-positive.
B) a glucose utilizer.
C) a major cause of venereal disease.
D) often carrying an antiphagocytic capsule.

E) None of the above
F) All of the above

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A

An organism isolated from a sputum sample that has a "hockey puck" consistency would have which of the following biochemical profiles?


A) Glucose-positive,maltose-positive,and lactose-positive
B) Glucose-positive,maltose-positive,and lactose-negative
C) Glucose-positive,maltose-negative,and lactose-negative
D) Glucose-negative,maltose-negative,and lactose-negative

E) All of the above
F) None of the above

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Gonococci require which of the following nutrients for growth?


A) Arginine
B) Hypoxanthine
C) Uracil
D) IsoVitaleX

E) A) and B)
F) All of the above

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MTM,Martin-Lewis (ML) ,and New York City (NYC) media were all developed for the selective isolation of which organism?


A) M.catarrhalis
B) Chlamydia trachomatis
C) N.gonorrhoeae
D) N.mucosa

E) None of the above
F) B) and C)

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The determination of carbohydrate utilization patterns for the differentiation of Neisseria spp.and M.catarrhalis is best performed by:


A) incubation of inoculated cysteine trypticase soy agar (CTA) with 1% dextrose,maltose,lactose,and sucrose for 48 to 72 hours.
B) oxidative-fermentative (OF) medium with dextrose,maltose,lactose,and sucrose.
C) heavy inoculation of buffered,low-peptone substrate with dextrose,maltose,lactose,and sucrose.
D) carbohydrate testing strips.

E) B) and D)
F) All of the above

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C

The second most commonly reported sexually transmitted infection in the United States,according to the CDC,is:


A) M.catarrhalis.
B) N.gonorrhoeae.
C) N.meningitidis.
D) HIV.

E) None of the above
F) All of the above

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The gram-negative coccus that can be described as oxidase-positive,glucose-negative,maltose-negative,sucrose-negative,lactose-negative,and DNase-positive is:


A) N.meningitidis.
B) M.catarrhalis.
C) Neisseria cinerea.
D) N.gonorrhoeae.

E) None of the above
F) A) and C)

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B

Gram-negative diplococci are isolated from a genital specimen and yield the following results: ·Glucose: positive ·Maltose: negative ·Lactose: negative The organism can be identified as:


A) N.gonorrhoeae.
B) Neisseria meningitidis.
C) Neisseria lactamica.
D) M.catarrhalis.

E) B) and C)
F) A) and C)

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N.gonorrhoeae is spread by:


A) person-to-person nosocomial spread by respiratory droplets.
B) person-to-person spread by sexual contact.
C) from infected mother to newborn during birth.
D) B and C only.

E) A) and B)
F) None of the above

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An advantage of all molecular assays is:


A) the ability to test for C.trachomatis from the same specimen at the same time.
B) the ability to use these tests for assessing the successfulness of treatment.
C) their role as evidence in medicolegal cases.
D) the ability to detect the capsular antigen in body fluids.

E) None of the above
F) B) and C)

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Which statement is true regarding the pathogenesis of N.cinerea?


A) N.cinerea is a leading cause of fatal bacterial meningitis.
B) Virulence factors include pili,capsule formation,and endotoxin.
C) Disseminated intravascular coagulation (DIC) and thrombocytopenia are complications of infection.
D) N.cinerea is commonly encountered as a contaminant in clinical specimens.

E) None of the above
F) B) and C)

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A gram-negative diplococcus grew on 5% sheep blood agar and chocolate agar,had scant growth on MacConkey agar,and had no growth on modified Thayer-Martin (MTM) agar.The organism was identified as N.sicca.Which one of the following statements explains the discrepancy of the results obtained on the MTM agar?


A) The medium was most likely expired.
B) The organism was most likely incorrectly transported.
C) The organism was most likely incubated at 35°C instead of 25°C.
D) No discrepancy is present.N.sicca does not grow on MTM agar.

E) B) and C)
F) All of the above

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Which one of the following statements is true regarding antimicrobial therapy and susceptibility testing?


A) Cephalosporins and macrolides are ineffective against M.catarrhalis.
B) Resistance to quinolones is emerging in strains of N.gonorrhoeae.
C) Most Neisseria spp.are resistant to penicillin.
D) Most strains of N.meningitidis are resistant to penicillin.

E) B) and C)
F) None of the above

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The differentiation of the similar M.catarrhalis and Neisseria can be best accomplished by:


A) examination of Gram stain of the colony.
B) vancomycin susceptibility testing.
C) the oxidase test.
D) carbohydrate utilization testing.

E) B) and D)
F) All of the above

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An endocervical swab was collected from a 26-year-old woman with a purulent discharge.The Gram stain revealed the following: ·Many white blood cells (WBCs) ·Moderate gram-negative diplococci ·Moderate gram-negative coccobacilli ·Few yeast Based on these findings,what would be the next course of action?


A) Report the isolation of Neisseria gonorrhoeae.
B) Work up the yeast.
C) Report the Gram stain,and follow up with culture.
D) Request a new specimen;this one is contaminated.

E) B) and D)
F) A) and D)

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Organisms belonging to the genus Neisseria are:


A) gram-positive diplococci.
B) gram-negative diplococci.
C) gram-negative coccobacilli.
D) gram-negative bacilli.

E) B) and C)
F) B) and D)

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M.catarrhalis can be described as a:


A) gram-positive diplococcus.
B) gram-negative diplococcus.
C) gram-negative coccobacillus.
D) gram-negative bacillus.

E) C) and D)
F) B) and D)

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M.catarrhalis may be differentiated from gonococci and meningococci on the basis of its:


A) growth on blood agar at 35°C.
B) growth on nutrient agar at 22°C.
C) reduction of nitrite to nitrate.
D) production of DNase.

E) All of the above
F) A) and B)

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Blood cultures collected for the recovery of N.gonorrhoeae or N.meningitidis require:


A) no special methods.
B) more than 0.025% sodium polyanethol sulfonate (SPS) .
C) transfer from Vacutainer tubes to culture broth within 1 hour of collection.
D) less than 0.025% SPS.

E) A) and B)
F) C) and D)

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