Question 1

You are shown a micrograph from a light microscope in which the specimens appear sharply focused and nearly three-dimensional. The micrograph is probably from a(n)  ________ microscope.

Accepted Answer

A)   dark-field
B)   phase-contrast
C)   Nomarski (differential interference contrast) 
D)   bright-field
E)   atomic forceF) A) and B)
G) None of the above

Question 2

Phage typing is useful for identifying bacteria because of the specificity of (antibodies/bacteriophages/PCR) for unique bacterial structures.

Accepted Answer

The answer of Phage typing is useful for identifying bacteria...

Question 3

Histological samples prepared with the ________ stain may reveal the presence of abnormal cells.

Accepted Answer

A)   acid-fast
B)   Gram
C)   simple safranin
D)   methylene blue
E)   hematoxylin and eosin (H
E)  F) B) and D)
G) A) and B)

Question 4

The resolution of a microscope lens is a function of the lens' (aperture/color/contrast).

Accepted Answer

The answer of The resolution of a microscope lens is...

Question 5

In the Schaffer-Fulton endospore stain, heat is a (fixation/mordant/staining) step.

Accepted Answer

The answer of In the Schaffer-Fulton endospore stain, heat is...

Question 6

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

Accepted Answer

A)   All cells would be purple.
B)   Gram-positive cells would be purple and Gram-negative cells would be colorless.
C)   All cells would be pink.
D)   Gram-positive cells would be pink and Gram-negative cells would be purple.
E)   Gram-positive cells would be colorless and Gram-negative cells would be pink.F) A) and E)
G) A) and D)

Question 7

Compare and contrast the light microscope with the electron microscope.

Accepted Answer

Both the light microscope and the electr...

Question 8

The function of a mordant in staining procedures is to

Accepted Answer

A)   fix the specimen to the slide.
B)   provide contrasting color.
C)   decrease the solubility of dye molecules.
D)   remove dye from certain structures.
E)   damage cellular structures so they take up dyes better.F) A) and B)
G) A) and C)

Question 9

The three domains proposed by Carl Woese and George Fox are the Archaea, the Eukarya, and the Protista.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following phenomena produces magnification?

Accepted Answer

A)   the wavelength of a radiation source
B)   the refraction of radiation as it passes through a lens
C)   the thickness of a microscopic specimen
D)   the numerical aperture of a lens
E)   the length of an objective lensF) A) and E)
G) A) and B)

Question 11

The ability of a lens to gather light is referred to as its

Accepted Answer

A)   resolution.
B)   numerical aperture.
C)   refraction.
D)   contrast.
E)   magnification.F) A) and D)
G) A) and E)

Question 12

Why does immersion oil improve resolution?

Accepted Answer

A)   It allows light to travel at a uniform speed on its way to the lens.
B)   It decreases the working distance.
C)   It increases the numerical aperture.
D)   It increases numerical aperture and maintains a uniform light speed.
E)   It increases the angle of refraction of the light.F) A) and C)
G) C) and D) D

Question 13

The Gram stain works because of differences in the ________ of bacteria.

Accepted Answer

A)   genetic characteristics
B)   cell walls
C)   cell membranes
D)   antigens
E)   capsulesF) B) and D)
G) D) and E)

Question 14

Low contrast specimens are made easier to see by

Accepted Answer

A)   increasing the amount of light passing through the slide.
B)   using dyes that react with their structures.
C)   adding color filters to the microscope.
D)   using smaller aperture lenses.
E)   drying them in a vacuum.F) B) and E)
G) B) and D)

Question 15

In Gram staining, ethanol-acetone is used as a

Accepted Answer

A)   decolorizing agent.
B)   counterstain.
C)   mordant.
D)   drying agent.
E)   primary stain.F) A) and B)
G) B) and D)

Question 16

Fluorescent dyes and lasers are used to visualize specimens on ________ microscopes.

Accepted Answer

A)   phase-contrast
B)   dark-field
C)   fluorescent
D)   confocal
E)   bright-fieldF) A) and E)
G) C) and E) D

Question 17

A serological test that involves the clumping of antigen and antibody is the (agglutination/antigen/ELISA) test.

Accepted Answer

The answer of A serological test that involves the clumping...

Question 18

The rules of naming organisms are called

Accepted Answer

A)   taxonomy.
B)   nomenclature.
C)   classification.
D)   binomials.
E)   identification.F) C) and E)
G) All of the above

Question 19

In a transmission electron microscope, the "lenses" are

Accepted Answer

A)   made of glass.
B)   thin films of metal.
C)   lasers.
D)   magnets.
E)   vacuums.F) C) and E)
G) A) and E)

Question 20

The part of the microscope indicated by the arrow in Figure 4.1 is the (ocular/objective/condenser) lens.

Accepted Answer

condenser

Exam 4: Microscopy, Staining, and Classification

You are shown a micrograph from a light microscope in which the specimens appear sharply focused and nearly three-dimensional. The micrograph is probably from a(n) ________ microscope.

Correct AnswerverifiedShow Answer

Phage typing is useful for identifying bacteria because of the specificity of (antibodies/bacteriophages/PCR) for unique bacterial structures.

Correct AnswerverifiedShow Answer

Histological samples prepared with the ________ stain may reveal the presence of abnormal cells.

Correct AnswerverifiedShow Answer

The resolution of a microscope lens is a function of the lens' (aperture/color/contrast).

Correct AnswerverifiedShow Answer

In the Schaffer-Fulton endospore stain, heat is a (fixation/mordant/staining) step.

Correct AnswerverifiedShow Answer

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

Correct AnswerverifiedShow Answer

Compare and contrast the light microscope with the electron microscope.

Correct AnswerverifiedBoth the light microscope and the electr...View AnswerShow Answer

The function of a mordant in staining procedures is to

Correct AnswerverifiedShow Answer

The three domains proposed by Carl Woese and George Fox are the Archaea, the Eukarya, and the Protista.

Correct AnswerverifiedShow Answer

Which of the following phenomena produces magnification?

Correct AnswerverifiedShow Answer

The ability of a lens to gather light is referred to as its

Correct AnswerverifiedShow Answer

Why does immersion oil improve resolution?

Correct AnswerverifiedD

The Gram stain works because of differences in the ________ of bacteria.

Correct AnswerverifiedShow Answer

Low contrast specimens are made easier to see by

Correct AnswerverifiedShow Answer

In Gram staining, ethanol-acetone is used as a

Correct AnswerverifiedShow Answer

Fluorescent dyes and lasers are used to visualize specimens on ________ microscopes.

Correct AnswerverifiedD

A serological test that involves the clumping of antigen and antibody is the (agglutination/antigen/ELISA) test.

Correct AnswerverifiedShow Answer

The rules of naming organisms are called

Correct AnswerverifiedShow Answer

In a transmission electron microscope, the "lenses" are

Correct AnswerverifiedShow Answer

The part of the microscope indicated by the arrow in Figure 4.1 is the (ocular/objective/condenser) lens.

Correct Answerverifiedcondenser

Correct Answer
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Both the light microscope and the electr...
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condenser