Question 1

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

Accepted Answer

A) True 
 B)False

Question 2

The proteome refers to all the protein molecules transcribed by the DNA.

Accepted Answer

A) True 
 B)False

Question 3

Random clones are regularly used to obtain the nucleotide sequences of isolated genes.

Accepted Answer

A) True 
 B)False

Question 4

In recombinant DNA technology, cutting different samples of a single DNA molecule with different restriction enzymes allows one to:

Accepted Answer

A)  amplify the DNA molecule.
B)  carry out DNA hybridization.
C)  locate a gene of interest.
D)  determine the function of a gene.
E)  determine the nucleotide sequence of a gene.F) A) and E)
G) A) and D)

Question 5

Genetically modified plants were first grown commercially in:

Accepted Answer

A)  1965.
B)  1985.
C)  1996.
D)  2001.
E)  2009.F) D) and E)
G) C) and D)

Question 6

The advantage of using the "ice-minus" strain of Pseudomonas syringae is:

Accepted Answer

A)  reducing susceptibility to fungi.
B)  increasing resistance to bacteria that thrive at low temperatures.
C)  increasing resistance to herbicides.
D)  reducing susceptibility to frost.
E)  increasing resistance to insect larvae.F) B) and C)
G) D) and E)

Question 7

In the Ti plasmid of Agrobacterium tumefaciens, the onc region contains genes that code for enzymes in:

Accepted Answer

A)  auxin synthesis only.
B)  opine synthesis only.
C)  cytokinin synthesis only.
D)  auxin and cytokinin synthesis.
E)  auxin and opine synthesis.F) B) and C)
G) C) and D)

Question 8

Totipotency refers to the ability of an individual plant cell to grow into an entire plant.

Accepted Answer

A) True 
 B)False

Question 9

Which of the following statements about prokaryotic genomes is FALSE?

Accepted Answer

A)  Most consist of a single circular chromosome.
B)  Approximately 90% of the identified genes have been assigned a function.
C)  About 25% of the genes have no significant similarity to other known genes in bacteria.
D)  Horizontal gene exchange periodically occurs.
E)  Gene transfers have occurred between bacteria and eukaryotes.F) B) and C)
G) A) and E)

Question 10

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Accepted Answer

A) True 
 B)False

Question 11

Nowadays, DNA sequencing is rarely done from specific restriction fragments.

Accepted Answer

A) True 
 B)False

Question 12

Agrobacterium induces the formation of crown-gall tumors by transferring the ____ plasmid containing ______ to the host plant's nuclear DNA.

Accepted Answer

A)  CG; CG-DNA
B)  O; R-DNA
C)  C; C-DNA
D)  Ti; T-DNA
E)  T-DNA; TiF) A) and E)
G) A) and D)

Question 13

In _________, electrical pulses cause pores in the plasma membrane to open, thus allowing DNA to enter the cell.

Accepted Answer

A)  particle bombardment
B)  the polymerase chain reaction
C)  a reaction involving reverse transcriptase
D)  electrophoresis
E)  electroporationF) B) and E)
G) C) and D)

Question 14

Which of the following statements concerning DNA libraries is FALSE?

Accepted Answer

A)  A genomic library is obtained by cutting the entire genome of an organism.
B)  A cDNA library is obtained by using reverse transcriptase.
C)  A DNA library contains cloned fragments of most of an organism's genome.
D)  A cDNA library lacks introns.
E)  A genomic library lacks exons.F) B) and E)
G) B) and C)

Question 15

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Accepted Answer

A) True 
 B)False

Question 16

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Accepted Answer

A) True 
 B)False

Question 17

One important characteristic the Taq polymerase is its

Accepted Answer

A)  insensitivity to pH.
B)  ability to glow when in the presence of a suitable substrate.
C)  ability to be cloned.
D)  resistance to high temperature.
E)  ability to transform cells.F) B) and D)
G) A) and B)

Question 18

Which of the following statements concerning vectors is FALSE?

Accepted Answer

A)  Vectors are used to transform cells.
B)  Vectors function as DNA carriers.
C)  A virus can be a vector.
D)  A plasmid can be a vector.
E)  A host cell can be a vector.F) A) and E)
G) B) and D)

Question 19

Most of the genes in all bacteria are significantly similar.

Accepted Answer

A) True 
 B)False

Question 20

Restriction enzymes cut single-stranded DNA molecules at or near their recognition sequences.

Accepted Answer

A) True 
 B)False

Exam 10: Recombinant Dna Technology, Plant Biotechnology, and Genomics

Which of the following statements about prokaryotic genomes is FALSE?

Correct AnswerverifiedShow Answer

Which of the following statements concerning DNA libraries is FALSE?

Correct AnswerverifiedShow Answer

Random clones are regularly used to obtain the nucleotide sequences of isolated genes.

Correct AnswerverifiedShow Answer

The advantage of using the "ice-minus" strain of Pseudomonas syringae is:

Correct AnswerverifiedShow Answer

Most of the genes in all bacteria are significantly similar.

Correct AnswerverifiedShow Answer

Totipotency refers to the ability of an individual plant cell to grow into an entire plant.

Correct AnswerverifiedShow Answer

Restriction enzymes cut single-stranded DNA molecules at or near their recognition sequences.

Correct AnswerverifiedShow Answer

When you grow a plant hydroponically, by definition you grow it without its roots in water.

Correct AnswerverifiedShow Answer

One important characteristic the Taq polymerase is its

Correct AnswerverifiedShow Answer

The proteome refers to all the protein molecules transcribed by the DNA.

Correct AnswerverifiedShow Answer

Which of the following statements concerning vectors is FALSE?

Correct AnswerverifiedShow Answer

Nowadays, DNA sequencing is rarely done from specific restriction fragments.

Correct AnswerverifiedShow Answer

Electroporation is used to transform monocots because Agrobacterium tumefaciens cannot infect them.

Correct AnswerverifiedShow Answer

In _________, electrical pulses cause pores in the plasma membrane to open, thus allowing DNA to enter the cell.

Correct AnswerverifiedShow Answer

Genetically modified plants were first grown commercially in:

Correct AnswerverifiedShow Answer

Agrobacterium induces the formation of crown-gall tumors by transferring the ____ plasmid containing ______ to the host plant's nuclear DNA.

Correct AnswerverifiedShow Answer

Horizontal/lateral gene exchange refers to related bacterial species exchanging genetic information over evolutionary time.

Correct AnswerverifiedShow Answer

In recombinant DNA technology, cutting different samples of a single DNA molecule with different restriction enzymes allows one to:

Correct AnswerverifiedShow Answer

The polymerase chain reaction is used to quickly make many copies of a DNA segment.

Correct AnswerverifiedShow Answer

In the Ti plasmid of Agrobacterium tumefaciens, the onc region contains genes that code for enzymes in:

Correct AnswerverifiedShow Answer

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Agrobacterium induces the formation of crown-gall tumors by transferring the plasmid containing __ to the host plant's nuclear DNA.

Correct Answer
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