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Saturation occurs when


A) there is so much enzyme present that the reaction rate decreases if more is added.
B) all enzymes are busy and further increases in substrate concentration will not increase reaction rate.
C) enzymes become fatigued and can no longer catalyze changes in the substrate.
D) intramolecular interactions are disrupted in the enzyme, decreasing its efficiency and decreasing reaction rate.
E) the substrate is denatured and the enzyme cannot catalyze any changes in its structure.

F) A) and B)
G) A) and C)

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Enzymes are biological catalysts that are crucial for normal human metabolism.

A) True
B) False

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During cellular respiration, decarboxylation occurs when


A) a carbon atom is removed from glucose.
B) a carbon dioxide molecule is removed from the cytosol.
C) a carboxyl group is released from pyruvate.
D) a carbon atom is removed from and an oxygen atom is added to fructose.
E) all carbon and oxygen atoms are released from a high-energy molecule.

F) A) and B)
G) B) and E)

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The enzyme DNA polymerase is synthesized


A) at a ribosome, and it remains within the cell.
B) at the rough endoplasmic reticulum, and it is secreted from the cell.
C) at the Golgi apparatus, and it is embedded in the cell membrane.
D) in the nucleus, and it is converted to RNA for export outside the cell.

E) A) and D)
F) B) and D)

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Which stage of cellular respiration is catalyzed by pyruvate dehydrogenase?


A) Electron transport system
B) Intermediate stage
C) Citric acid cycle
D) Glycolysis
E) Fermentation to lactate

F) C) and D)
G) B) and C)

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Cold temperatures (below 36°C) denature proteins, which is why biochemistry labs never freeze enzymes.

A) True
B) False

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A reaction with a very high activation energy should have a very high reaction rate.

A) True
B) False

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Imagine that you discovered an enzyme in the human stomach that turned the substrate "ABC" into the product "XYZ". Name this enzyme (using the standard convention) and describe its chemical characteristics. Under what conditions would it be an effective catalyst? How might its activity be regulated?

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The enzyme "ABCase" would work best at o...

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A group of enzymes that are physically attached to one another is referred to as a(n)


A) allosteric pathway.
B) metabolic pathway.
C) multienzyme complex.
D) phosphate complex.

E) A) and B)
F) None of the above

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In performing its enzymatic function, carbonic anhydrase


A) requires the inorganic coenzyme zinc.
B) requires the organic coenzyme carbon dioxide.
C) is facilitated by the inorganic coenzyme NAD+.
D) is facilitated by the organic coenzyme zinc.
E) requires the inorganic coenzyme NADPH.

F) B) and D)
G) A) and B)

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The enzyme-substrate complex is


A) another name for the active site.
B) the reactants whose chemical reaction the enzyme catalyzes.
C) the chemical structure formed when the substrate binds to the active site.
D) a pocket on the substrate that the enzyme recognizes.

E) All of the above
F) None of the above

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During electron transport, how many ATP can be generated from the energy contributed by a molecule of NADH?


A) 1
B) 2
C) 3
D) 30
E) 36

F) D) and E)
G) A) and C)

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A protein kinase is an enzyme that adds a phosphate to its substrate.

A) True
B) False

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Which one of the following choices includes three descriptions that are consistent with each other (that is, that describe the same type of chemical reaction) ?


A) Anabolic, dehydration, endergonic
B) Exchange, hydrolysis, endergonic
C) Catabolic, dehydration, irreversible
D) Metabolic, synthesis, exergonic
E) Anabolic, catabolic, dehydration

F) C) and D)
G) A) and B)

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The first law of thermodynamics states that once energy is consumed, it can no longer be converted to any other form of energy.

A) True
B) False

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A fever of 101°F causes most enzymes in the human body


A) to denature.
B) to become more rigid.
C) to be more flexible and efficient.
D) to saturate.

E) None of the above
F) B) and D)

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In the final step of enzymatic catalysis,


A) the substrate is released and the enzyme is inactivated.
B) the product is completed and the enzyme is inactivated.
C) the products are released and the enzyme is free to bind other substrates.
D) the enzyme and substrate form chemical bonds with each other resulting in a new product.
E) equilibrium is obtained so that the enzyme does not catalyze further reactions.

F) All of the above
G) B) and D)

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Decarboxylation occurs during the intermediate stage of aerobic cellular respiration.

A) True
B) False

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The intermediate stage of cellular respiration


A) produces ATP and occurs in the cytosol.
B) produces carbon dioxide and occurs in the mitochondria.
C) requires Coenzyme A and occurs in the cytosol.
D) produces pyruvate and occurs in the mitochondria.

E) A) and B)
F) A) and C)

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Which of the following choices lists the order of stages of cellular respiration in proper order?


A) Glycolysis, intermediate stage, citric acid cycle, and the electron transport system
B) Citric acid cycle, glycolysis, intermediate stage, and the electron transport system
C) Glycolysis, citric acid cycle, intermediate stage, and the electron transport system
D) Glycolysis, intermediate stage, electron transport system, and the citric acid cycle
E) Citric acid cycle, electron transport chain, intermediate stage, and glycolysis

F) None of the above
G) A) and E)

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