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Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

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Question 21

Multiple Choice

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Microbes can be challenging to study because they often grow in complex communities and it may be difficult to separate the various species from one another to study individually. Which of the following laboratory "tools" or techniques may be of the the most help with separating out different species from complex mixtures?

A) use of transmission electron microscopy
B) use of enriched agar for culture
C) use of selective agars for culture
D) use of light microscopy

E) B) and C)
F) None of the above

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Question 22

Multiple Choice

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Which of the following media is useful for cultivating fungi?

A) Sabouraud's agar
B) MacConkey agar
C) tomato juice agar
D) phenylethanol agar
E) Mueller tellurite

F) A) and E)
G) All of the above

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Question 23

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One colony typically develops from the growth of several parent bacterial cells.

Bacteria that require special growth factors and complex organic substances are called

All of the following are examples of basic dyes except

A student was given a stained smear to observe. Upon observation, the student noted rod-shaped bacteria that were clear and unstained against a darker background. What sort of stain was performed on this slide?

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

The three physical forms of laboratory media are: solid, semisolid, and _____.

Each of the following are characteristics of microorganisms that pose unique challenges to their study EXCEPT

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

The Six "I's" of studying microorganisms include all of the following except

_____ is the term for a starting a new culture from an existing one.

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Media that contains extracts from plants, animals, or yeasts are

To prepare large volumes of influenza virus for use in the flu vaccines each year, the virus is grown in embryonated hen eggs. This is an example of the use of

Which microscope does not use light in forming the specimen image?

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

You are doing research on a bacterial species, trying to determine the shape, distribution, and arrangement of a number of filamentous structures that protrude from the cell surface. Which type of microscopy would best enable you to do this?

Syphilis is diagnosed either with microscopy or serology because the causative agent Treponema pallidum) does not grow on any microbiological media. Which term best describes this aspect of the T. pallidum?

At the end of the Gram stain, gram-positive bacteria will be seen as purple cells.