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A __________ effect is an observable change that occurs in cells as a result of viral replication.

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Determining the susceptibility of a microorganism to specific chemotherapeutic agents is an important service performed in the clinical microbiology laboratory.

A) True
B) False

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The immunologic technique involving the transfer of proteins separated by electrophoresis to nitrocellulose sheets is


A) complement fixation.
B) direct immunofluorescence.
C) immunoblotting.
D) immunoelectrophoresis.

E) B) and C)
F) B) and D)

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Which of the following media will permit the growth of one group of bacteria while inhibiting the growth of some other groups?


A) differential
B) selective
C) enrichment
D) characteristic

E) A) and B)
F) None of the above

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The use of rRNA gene sequences to identify bacterial strains is referred to as ___________.

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A colorless substrate acted on by the enzyme portion of the ligand to produce a colored product is known as a


A) antibody.
B) chromogen.
C) antigen.
D) serotype.

E) All of the above
F) A) and B)

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If one is looking for intracellular inclusion bodies,one would suspect the microorganism to be a


A) mycoplasma.
B) rickettsia.
C) chlamydia.
D) protozoan.

E) A) and C)
F) A) and D)

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In the clinical laboratory,which of the following is (are) used as a rapid method of identification?


A) Manual biochemical kit systems.
B) Mechanized/automated systems.
C) Immunologic systems.
D) All of the choices.

E) C) and D)
F) None of the above

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Hybridoma cells and their progeny normally produce a(n)


A) monoclonal antibody.
B) polyclonal antibody.
C) radioisotopes.
D) antigens.

E) A) and D)
F) B) and C)

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The capsular swelling that occurs when Streptococcus pneumoniae is treated with an antiserum for its specific capsular type is called the __________ reaction.

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The immunological reaction that occurs when there is an optimal ratio of antigen and antibody,producing a lattice at the zone of equivalence is known


A) immunoblotting.
B) immunoelectrophoresis.
C) immunodiffusion.
D) immunoprecipitation.

E) A) and B)
F) A) and D)

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Which of the following tests is an agglutination test for the bacterium causing typhoid fever?


A) Quellung reaction
B) API 20E system
C) Widal test
D) IMViC

E) A) and B)
F) C) and D)

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Identification and characterization of ova,trophozoites,and cysts of parasites is typically accomplished by direct microscopic evaluation of the clinical specimen.

A) True
B) False

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Which of the following is not used to culture viruses?


A) Growth on artificial media.
B) Growth in cell cultures.
C) Growth in embryonated hen's eggs.
D) Growth in whole animals.

E) C) and D)
F) B) and C)

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DNA probe technology identifies microorganisms by probing their genetic composition.

A) True
B) False

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Which of the following should be isolated only by specialized research laboratories?


A) mycoplasmas
B) rickettsias
C) chlamydiae
D) all of the choices

E) A) and B)
F) A) and C)

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Detection of single or multiple microorganisms based on their cell size is known as


A) flow cytometry.
B) complement fixation.
C) agglutination.
D) serotyping.

E) All of the above
F) A) and D)

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PCR is considered more flexible and easier to modify than antibody-based identification technologies.

A) True
B) False

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Which of the following cell cultures consists of cells derived directly from kidney tissues?


A) Continuous cell cultures.
B) Semicontinuous cell cultures.
C) Primary cultures.
D) All of the choices.

E) A) and C)
F) A) and B)

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Which of the following is useful in the identification of bacteria?


A) Source of the culture specimen.
B) Growth patterns on selective and differential media.
C) Hemolytic,metabolic,and fermentative properties.
D) All of the choices.

E) A) and B)
F) A) and C)

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